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Resource Guide

Sample Submission Guide

Best practices for collection, storage, and shipping to ensure the highest quality results.

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Cells & Tissue

  • Snap-freeze in liquid N2 within 5 min of collection
  • Store at -80°C; ship on dry ice
  • Min of 1e6 cells or 5 mg tissue per replicate
  • Avoid repeated freeze-thaw cycles

Biofluids (Plasma / Serum)

  • Collect in EDTA or heparin tubes
  • Centrifuge at 2000g for 10 min at 4°C
  • Aliquot and store at -80°C; ship on dry ice
  • Min 50 µL per replicate for standard proteomics

FFPE Blocks

  • Provide H&E-stained section for pathologist review
  • Min block size: 2 mm core
  • Avoid decalcification if possible
  • Include matched frozen tissue when available

DNA/RNA (Sequencing)

  • Extract using column-based purification (Qiagen recommended)
  • Ship in RNAstable or DNAstable tubes at ambient
  • Min: 100 ng DNA (WGS), 50 ng RNA (RNA-seq)
  • Provide RIN score ≥ 7 for RNA

Note: These are general guidelines. We provide sample-type-specific protocols and collection kits upon request. Contact us for custom shipping arrangements.

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Further reading

  1. Wiśniewski JR, Zougman A, Nagaraj N, Mann M. Universal sample preparation method for proteome analysis. Nat Methods 2009;6:359-362. PubMed · DOI
  2. Zougman A, Selby PJ, Banks RE. Suspension trapping (STrap) sample preparation method for bottom-up proteomics analysis. Proteomics 2014;14:1006. PubMed · DOI
  3. Hughes et al. Single-pot, solid-phase-enhanced sample preparation for proteomics experiments. Nat Protoc 2019. PubMed · DOI

Citations verified against PubMed records; “Open access” marks papers whose full text is freely available via PubMed Central or the journal.